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'Colony 48 h' young bees that experienced their first 48 h in a field colony and then monitored individually in constant laboratory conditions.
The light exposure lasted less than a minute during the introduction to the field colony and 5 10 min during collection.
We assigned newly emerged bees to one of the following treatments: (i) 48 h in a field colony ('Colony 48 h'); (ii) 24 h in a field colony ('Colony 24 h'); (iii) 48 h isolated in a Petri dish provisioned with sugar syrup (50% w/v) and pollen, and placed in the laboratory (31±1 °C; RH=55±5%, 'Lab individually 48 h').
To collect the cohort of foragers we blocked the entrance to the source field colony using a metal 8-hole per inch mesh and caught >1,500 foragers returning to the hive with pollen loads on their hind legs.
In Experiments 1 and 6, we established triple-cohort colonies with newly emerged bees (0 24 h of age), nurses, foragers (each cohort consisted of >1,500 bees) and their mother queen, all collected from the same source field colony (so all individuals were genetically related).
Because eggs or workers originating from the same field colony were sometimes used twice in the analyses (albeit once per egg origin), we included the colony of origin of eggs and workers as random factors in all the analyses.
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The only factor controlled was light intensity, and our main conclusion was that overall corals on the platform became darker than field colonies in response to shading, but adjusted back to the same color level as field colonies after shade removal.
In Experiments 2 5 the bees that were introduced to field colonies were exposed to daylight twice (during the introduction and the collection from the hive); thus, we also exposed the bees from the other treatments to a similar daylight experience.
As interactions inside the mesh enclosures may have synchronized the bees in Experiment 4, we performed a complementary experiment (Experiment 5) in which we caged bees individually in SM or DM enclosures inside field colonies.
To obtain newly emerged bees, we removed honeycomb frames with sealed brood from field colonies, brushed off all adult bees and immediately placed the frame inside a lightproof box.
The extent to which the small tents of the early instars of Malacosoma americanum warmed when irradiated by sunlight was investigated by continuous electronic monitoring of field colonies for 137 colony-days.
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