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Animal models of human behavioral endophenotypes, such as the Tail Suspension Test (TST) and the Open Field assay (OF), have proven to be essential tools in revealing the genetics and mechanisms of psychiatric diseases.
Some of the mice from cohort 2 (12/gender/genotype) were subsequently tested (at 5 months) in the open field assay (OF) to determine whether possible changes in activity levels could contribute to the effects observed in the hot plate test.
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The pea spring variety Solara (susceptible) was used as the adjacent control in the field assays of the RIL population.
The study was used to confirm the diagnostic performance of a rapid and simple field assay for the serodiagnosis of brucellosis in cattle [ 17].
For the Open Field assay, the movement of P15 mice was recorded during 10 min in a square, white Plexiglas box.
Briefly, in the open field assay the movements of the mice in a 40 cm × 40 cm box, with 25 cm × 25 cm defined as the center, were tracked for 30 min and analyzed for total distance traveled and duration and distance traveled in the center using AccuScan Fusion software (Omnitech, Columbus, OH).
Existing techniques in the detection of ALK IHC protein and ALK gene copy number alterations need to be refined as is evident from the elegant work done by Nitta et al (2013) who developed a dual bright-field assay for concurrent detection of ALK protein expression and ALK gene rearrangement with much more sensitivity.
It has great value for determining the severity and prognosis of bacterial infection and providing guidance for the usage of antibiotics, especially in the case of emergency or field assay.
Importantly, this portable field assay allows for the economic usage of limited biological material (parasites and reticulocytes) and the intracellular labeling of the target cells reduces the need for highly purified schizont inoculums.
In the open field assay there was a significant effect of genotype on the total distance traveled in the open field [F 2, 60) = 4.192, P<0.02] and number of rears (vertical movements) [F 2, 60) = 9.14, P<0.001] (Fig. 3A, B).
A review of field assays conducted worldwide18 found that all reports of assays conducted in Brazil and Mexico described positive yield-enhancements by Azospirillum.
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