Sentence examples for fidelity of ligation from inspiring English sources

Diagnostic restriction digests with EcoRI [69] were performed to check the fidelity of ligation.

The identity of the tethered mononucleotide and its pairing partner in the bridging oligo strongly affected kinetic rate constants for adenylate formation (k2) and gap-sealing (k3), and thereby determined the overall efficiency and fidelity of the ligation reaction.

Prp22 mutants that reduce ATPase activity compromise the fidelity of exon ligation both in vivo and in vitro [ 192].

The in vitro ligation activity assay followed three steps: (1) annealing of oligomers, (2) ligating of the nick, and (3) detection of ligation.

The type of ligation may have an effect.

Figure 5 Influence of type of ligation on friction.

In addition, the positive mutation detection was achieved with a wild-type to mutant ratio of 10��000:1, due to the high fidelity of DNA ligase in differentiating mismatched bases at the ligation site.

The limitations of this method are low fidelity of Taq DNA polymerase causing unwanted mutations and requirement of subcloning into the final target vector with restriction digestion and ligation.

Craig Colclough captured the noble fidelity of Kurwenal.

D.K. calculated the read-out fidelity of the device.

The length, complexity, and fidelity of oral traditions varies enormously.