FIC of H1, H2, H3=MIC of H1, H2, H3, respectively, in combination with antibiotic/MIC of H1, H2, H3 alone; FIC of antibiotics=MIC of antibiotic in combination with peptide/MIC of antibiotic alone; FICI = FIC of H1, H2, H3+ antibiotic, respectively.
The suma of both FICs in each well was used to classify the combination of antimicrobial agents as synergistic effect when FIC indexes were ≤ 0.5; partial synergy FIC >0.5 but < 1; additive FIC = 1.0; indifferent effect when values were >1 and < 4 and antagonistic when values were ≥ 4.0 [ 18].
In the first step for all 816 triplets, a set of 13 equally fractionated concentrations, from FIC A 0.1 + FIC B 0.1 + FIC C 0.1 to FIC A 1 + FIC B 1 + FIC C 1, were tested in the model, thus yielding ∑FICs from 0.3 to 3. Of these, 360 triplets that resulted in the inhibition of growth at a ∑FIC of <1.2 were advanced for an exhaustive analysis of synergy.
Specifically, the lowest bias of each method was 0.09 by δ with 100 AIMs, 0.005 by FST with 50 AIMs, 0.15 by FIC with 20 AIMs, 0.07 by SIC with 50 AIMs, and 0.01 by In with 50 AIMs.
A combined effect was defined according to the fractional inhibitory concentration (FIC) index deduced from MIC values, as follows: synergistic effect, FIC ≤ 0.5; additive effect, FIC 0.6 1.0; no effect, FIC 1.1 2; and antagonistic effect, FIC > 2.
A special panel of global health experts who are more likely to understand the logistical constraints and nuances of conducting global health research review the FIC K01 awards [23].
We identify several additional HTH folds among domains of unknown function fused to fic (duf977, duf1612, and duf2089 in Figure 3).
Given the similar overall fold and the common motif positioned between corresponding α-helices, doc was suggested to have evolved from fic [10].
Although the AvrB sequence lacks several residues of the fido motif, a conserved Arg (R117 in P. syringae AvrB) that corresponds to fic R50 maintains a similar local structure in the AvrB loop (Figure 4C).
These two amounts equate to the total cost for vaccines for 122 FIC (7.6% of FIC during the period).
Eighteen primer pairs were used to perform 5 multiplex and 3 simplex PCRs, which recognized FIA, FIB, FIC, HI1, HI2, I1-Iγ, L/M, N, P, W, T, A/C, K, B/O, X, Y, and FII replicons (4 ).
