In fact, replacing the optical fibre of our microscope head by a 10 μm diameter cylindrical capillary, we succeeded in local collection of sample XRF under X-ray illumination using an in-lab source.
A 200 μm core fibre connects the microscope to a CCD UV/Vis spectrometer AvaSpec-2048TECC, Avantes BV).
Such investigations were made by dissecting single nerve fibres under the microscope and placing them on electrodes or by inserting very fine wires (e.g., tungsten microelectrodes) directly into the intact nerve.
On arrival at Cardiff University all fish were briefly anaesthetised in 0.02% MS222 and externally screened for visible parasites under a stereo-microscope with fibre optic illumination.
Samples (5 mm long × 2 mm wide) were cut from bast fibre strips under a stereo microscope and fixed in 3% v/v glutaraldehyde, +2% paraformaldehyde in 0.1 M Na-cacodylate buffer (pH 7.2).
With this study it is intended to achieve a more realistic characterization of the stresses maximum values for a set of case studies, through the use of fibre geometry data obtained from microscope images of carbon fibre laminated composites.
Viewed under a microscope, her muscle fibres, rather than being neatly arranged in rows, are frayed and disorganized.
Visual inspections of the rope and yarns including scanning electron microscope analysis of fibres revealed that abrasion wear was accelerated by debris found within the rope structure, highlighting the importance of preventing particle ingress.
Knowledge on the microdistribution of hemicelluloses (i.e. xylans) within wood cell walls has come primarily from early gross chemical studies on sections from cambium layers at different stages of cell wall differentiation [ 4], by the application of xylanase-gold complexes to plant cell walls and pulp fibres [ 5- 7] and by microscope observations after selective xylan removal [ 8, 9].
To analyze the SDH activity in muscle fibres a Nikon Microphot FXA microscope (Nikon Instruments Europe BV, Badhoevendorp, The Netherlands) and the Lucia-G analyzing software (Laboratory Imaging, Prague, Czech Republic) were used.
