The focussed response required a metabolisable resource, as only limited changes were seen with glass fibre discs used to mimic the osmotic and thigmotropic stimuli upon resource addition.
Sterile 7 mm glass fibre discs containing 10 μl essential oil were added to each disc.
An absence of antibacterial activity would produce a halo of 7 mm diameter, the size of the glass fibre discs.
The precipitate S-GAG-cetyl pyridinium chloride complexes were collected by filtration through glass fibre discs using a cell harvester (Skatron Instruments AS).
Sterile 7 mm glass fibre discs (Whatmann GF-C, Sigma, Poole, Dorset, UK) were immediately placed on the surface of the bacterial plates and essential oil (10 μl) was added to each disc.
In order to investigate whether the essential oils could lyse E. coli that were already present rather than inhibiting their growth, zone of clearance assays were conducted, ie glass fibre discs containing essential oils were placed on pre-existing E. coli lawns, and halos were allowed to develop.
However, while the range of equipment listed on the websites includes the distinctive red helmets used by Team GB in the velodrome, and the special aerodynamic wide forks that feature on their track bikes, one item does not appear: carbon-fibre disc wheels, indicating that contrary to French doubts, Great Britain could well be using standard kit.
Titanium fibre mesh discs with or without a thin HA coating were implanted into rat cranial bone defects, and the animals were killed at 2 and 4 weeks.
Muscle fibres, z-discs and mitochondria are resolved (Fig. 3), neuronal synapses can be observed in the NT (data not shown) and structural detail of pigment cells and scales are evident (Fig. 5).
For validation, a case study was performed where the thermal analysis by laser flash of a carbon fibre composite-copper disc was simulated such that computational and experimental results could be compared directly.
Automated perimetry, tonometry, corneal pachymetry, imaging of the optic disc, nerve fibre layer and anterior segment, may all generate digital outputs that can be transferred electronically and viewed remotely.
