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(D ) Representation of the 'unstable' and 'stable' dorsal stress fiber zones in U2OS cells.
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In intact animals type II-collagen was immunodetected in all zones of unmineralized and mineralized articular cartilage, while immunostaining for this protein was negative to weak in the deep radiated fiber zone of ovariectomized minipigs.
In the equator, a row of large nuclei aligned in the fiber zone underneath the epithelial layer (Fig. 3Cb).
For analysis, 4 different areas in the cortical lens fiber zone close to the OFZ were randomly selected.
A disorganization of the postequatorial fiber zone was also observed, which has been reported in human posterior subcapsular cataracts.
The capsule is attached to the lens fiber zone inside by the adhesive molecule laminin (Hiraoka et al., 2013).
Then the cell could have changed direction toward the fiber zone underneath, converted into a lens fiber cell and accumulated in a layer.
These nuclei are lined obliquely in a row on the inward extension in the new fiber zone and they disappear as the lamellar organization develops.
In contrast, the lens-specific water channel protein AQP0/aquaporin-0 remained highly expressed at the membranes of differentiated lens fiber cells in the central lens fiber zone (see Fig. S2).
The width of lighter purple staining with aldehyde-fuchsin demonstrated wider interspaces in the anterior fiber zone (Fig. 3Ca) than the posterior zone (Fig. 3Cc), suggesting differences in flexibility.
Suppressing MTOR activity in these lenses induced the premature loss of organelles from lens fiber cells, as shown both by immunostaining and immunoblotting for the ER marker HSPA5 in the central fiber zone (Fig. 6C).
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