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A first type of putative tail fiber protein is essentially composed of only the conserved DUF3751 domain ("a-type" tail fiber; supplementary table S3, Supplementary Material online).
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In K6W-Ub expressing lenses the thick layer of disorganized cells is positive for connexin 43 indicating that these cells are epithelial an not degenerating fibers (Supplementary Fig S2A, 2B).
The incomplete switch in fiber type transition from glycolytic to oxidative fibers in our experiments is also supported by only partial changes in the positioning of the Golgi complex and microtubules in the converted fibers (Supplementary Material, Figures S1 and S2).
In contrast, PLN was barely detectable in some WT type I and type IIA fibers (supplementary material Fig. S1).
In regenerated muscle, H2B-GFP+ SCs contribute to the myonuclei of regenerated muscle fibers (supplementary material Fig. S2D,E).
Conversely, structural elements of excitation contraction coupling and glycolysis defined a cluster highly intense in 2B fibers (Supplementary Fig S3C).
They were located both at the myosepta and scattered throughout the muscle fibers (Supplementary Movie S5) and were not observed in control embryos (Supplementary Movie S6).
Other TCA cycle enzymes, such as malate dehydrogenase (Mdh2), were also significantly more highly expressed in 2X fibers (Supplementary Table S3).
On staining type I and type II fibers (Supplementary Figure 3) we found no differences between hLPL transgenic and wt mice.
In addition, we devised a strategy to follow H2B-GFP levels in terminally differentiated nuclei of mature muscle fibers (supplementary material Fig. S2I).
However, the atrophic fibers with internal nuclei in the Lmod3 PB/PB TA muscles were negative when stained with an embryonic myosin heavy chain antibody, a marker for active regenerating fibers (supplementary material Fig. S4A-E).
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