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A parametric study including different fiber sizes, fiber contents and ages of the reinforced render was carried out.
Using electrically aligned scaffolds, fibroblast directionality deviations can be maintained in the range of nanofiber alignment deviation for fiber sizes down to ∼100 nm.
Fiber sizes smaller than chondrocyte size, and widely spaced, are therefore beneficial in terms of improved adhesion by cell shape adaptation.
The ability to produce micro/nano materials with various compositions, fiber sizes and fiber arrangements make electrospinning a versatile technique for fabricating biomimetic structures for tissue engineering applications.
Hematoxylin and eosin (H&E) staining showed the large areas of degenerating fibers, high variation in fiber sizes, and high percentage of centrally nucleated fibers (CNFs) in the skeletal muscles of the untreated P448L mice (Fig. 4a, Supplementary Figure 4a, and Fig. 5).
Different fiber sizes were obtained by varying the concentration of polyacrylonitrile solution (namely 4, 6, 8 and 10 wt%) to explore the interplay between electrospun fiber size and rejected salt ions.
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Ohira, Y. et al. Gravitational unloading effects on muscle fiber size, phenotype and myonuclear number.
No modifications of fiber size were observed in females.
Edgerton, V. R. et al. Human fiber size and enzymatic properties after 5 and 11 days of spaceflight.
Notably, improvement in pathology with reduced infiltration and fiber size variation was also observed in the diaphragm (Supplementary Figure 8).
Treatment with ribitol improved the dystrophic pathology of limb muscles as evidenced by the diminished foci of necrotic fibers and a more homogenously distributed fiber size.
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