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Actual commercial fiber, sized to rat-respirable dimension, having an average fiber diameter of 1 micron and an average length between 15 and 25 microns, was used in the experiments.
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Immunohistochemistry was employed to determine the fiber sizes, fiber number and myosin heavy chain (MHC) composition of examined muscles as described previously [12].
Remarkably, distribution of muscle fiber sizes for AAV6:miR SOD1 and AAV6 + AAV9 miR SOD1 groups largely matched that of WT mice.
The highest transmission attained is about 71% for fiber sizes less than the shank cross-sectional area.
Ohira, Y. et al. Gravitational unloading effects on muscle fiber size, phenotype and myonuclear number.
No modifications of fiber size were observed in females.
Edgerton, V. R. et al. Human fiber size and enzymatic properties after 5 and 11 days of spaceflight.
Notably, improvement in pathology with reduced infiltration and fiber size variation was also observed in the diaphragm (Supplementary Figure 8).
Treatment with ribitol improved the dystrophic pathology of limb muscles as evidenced by the diminished foci of necrotic fibers and a more homogenously distributed fiber size.
Fiber size increased proportionally with increasing protein and polymer concentrations, whereas pore size decreased.
Elucidating the molecular signals that allow for increases in muscle fiber size or endurance has implications for the treatment of chronic diseases ranging from sarcopenia to diabetes.
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