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Direct 2D and 3D images were generated in which individual fibers were clearly resolved, along with portions of the foam structure.
The fiber-optic microscope resolved individual nuclei in all specimens and tissues imaged.
In order to obtain accurate fiber orientation from histology images, a 40 × objective lens was used to image the histology samples so that individual fibers can be resolved.
It cannot be ruled out that strongly ordered cell wall macrofibrils from other cell types, like sclerenchyma fibers, would be resolved by 3D-SIM.
We used the same system as described above, but now with a lower density of actin such that single fibers could be resolved by confocal microscopy.
When regions of this data set are shown at higher magnifications, individual fibers and fiber microstructure can be clearly resolved (Figs. 5B, D and Fig. 5D, inset).
Using the birefringence property of myelin sheath, nerve fiber tracts as small as a few tens of micrometers can be resolved and neighboring fiber tracts with different orientations can be distinguished in cross-sectional optical slices, 2D en-face images and 3D volumetric images.
The mLCI instrument uses multiple delivery fibers to acquire depth resolved reflection profiles across large scanned tissue areas.
A modification of the method of microfluidic stretching of DNA is required to provide better resolved DNA fibers.
This system acquires the intensity spectra coming simultaneously from the 6 collecting fibers, allowing for spatially resolved measurements.
For accurate estimation of diffusion restrictions, the discrepancy between results for cardiomyocytes and those for fibers has to be resolved.
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