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The contest set off a flood of gimmicky efforts to attract the company's attention – from Topeka, which changed the name of its city to Google for a month, to Madison, Wis., which created a "Google Fiber" ice cream flavor.
Not to be outdone, a dairy in Madison, Wis., aided that city's efforts by creating a "Google Fiber" ice cream flavor — vanilla ice cream with granola and M&Ms to match Google's multicolored logo.
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This paper reports on a novel fiber-optic ice sensor featuring oblique fiber end-faces, not perpendicular to the fiber axis.
To permeabilize the myofibers, we incubated each fiber bundle in ice-cold buffer X containing 50 μg/ml saponin on a rotator for 30 min at 4°C.
Samples were filtered onto 47-mm glass fiber filters and shipped on dry ice.
After the incubation, samples were immediately filtered through glass fiber filters and washed twice with ice-cold HEPES buffer.
After incubation, the membranes were filtered through glass fiber filters and washed three times with ice-cold 5 mM Tris-HCl buffer on a Brandel cell harvester.
These organic reinforcements include fibers that strengthen the ice and regulate its melting time.
For each replicate, about 200 mg of frozen fibers were thawed on ice.
Muscle fibers were added to ice cold saponin/BIOPS and gently agitated for 30 minutes.
After skinning, the fibers were stored on ice in skinning solution without Triton X-100 over night.
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