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Each experimental caterpillar was confined to its own closed 162.7 ml clear plastic SOLO brand soufflé cup with the weighed, dry glass fiber disc impaled on a pin pushed through one side of the cup.
Eighty final instar caterpillars that appeared to be still feeding were then randomly divided into two no-choice feeding treatments: a glass fiber disc treated with either 0.01 mM monocrotaline (PA) or 1.0 mM sucrose in distilled water.
To calculate the mass of each disc consumed during the assay (amount eaten), the final dry mass of each glass fiber disc was subtracted from its initial dry mass.
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The no-choice feeding experiment quantified the ingestion of glass fiber discs treated with PA or sucrose, in isolation from other chemicals, by a set of field-collected caterpillars that included naturally parasitized and unparasitized individuals.
The polybrene-coated glass fiber discs were used for sample immobilization according to the manufacturer's instructions.
The peptides were then sequenced using a Procise™ protein sequencer (Model 491 HT; Applied Biosystem, Warrington, UK) in the gas phase mode from Biobrene™-treated glass fiber discs.
Special brakes with six-piston calipers biting carbon-fiber discs bring the car to a stop well before the Nevada state line.
This sequence-dependent sensitivity of CMP assembly has been observed many times before: increasing core POG repeat length of metal-ligated CMP assemblies results in shifts from meshes to florettes to saddle-like structures and increasing the number of core bipyridines lead to the formation of fibers, discs and then to hollow spheres.
The one forth segment of fiber glass disc is kept transparent and rest portion is covered up by a semi-transparent layer.
Composite pore filled membranes formed by impregnating glass fiber filters disc with the polymer mixture prior to gelation were used to determine the transport changes in response to temperature, using riboflavin (MW: 376), reactive red 120 (MW: 1470) and lysozyme (MW: 14300).
Mainstream smoke was passed through a 92-mm glass fiber filter disc for particulate matter collection.
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