Early genotyping of HAdV-D53 by sequencing of the hexon (the major neutralization determinant) and other determinants (fiber and penton) gave results of a recombinant strain HAdV-D22/H8 [19].
Although in vitro bioassays cannot evaluate characteristics such as clearance and/or durability of fibers which may be critical determinants of fiber toxicity in lung, they can be used both to address dosimetry at the cellular level (i.e., number of fibers per cell that elicit a measurable biologic end point) and to evaluate preventive approaches to fiber-induced cell injury.
DNA polymerase, penton base (β-determinant), pVII, protein V, L1 and L2 of the hexon, and fiber knob (γ-determinant) nucleotide sequences were compared by sequential pairwise alignment with the Clustal Algorithm implemented in the BioEdit software package (version 6.0.5) and adjusted manually to conform to the optimized alignment of deduced amino acid sequences.
Fibrillin microfibrils and elastic fibers are critical determinants of elastic tissues where they define as tissue-specific architectures vital mechanical properties such as pliability and elastic recoil.
Thus, HAdV-D53 fulfilled the hexon L1 and L2 criteria for typing as HAdV-D22 [18], with a fiber knob (hemagglutination determinant) sequence identical to HAdV-D8.
The graphical alignments showed the close relationship between HAdV-A31 and -A12 with the exception of the coding regions for immunogenic determinants (hexon, fiber).
Furthermore, how fibers cause diseases and what specific determinants are critical to fiber-induced toxicity and carcinogenicity are still not completely understood.
Cell cellulose fiber interaction is the determinant signal in the cell biomaterial responses, isolated from other frequently present interferences such as protein and other chemical traces usually present in cell culture matrices.
The irregular and wavy aspect of crystals induced by the substitution mutant Ad5F497D might let suppose that the F residue of the highly conserved motif FMP motif at position 497 499 in Ad5 fiber was a major determinant of the crystal structure (Fig. 7 A, B).
Bacteria and their metabolites can have direct effects on colonocytes via barrier dysfunction, epithelial proliferation, inflammatory, DNA damage, genotoxic ways, etc. Dietary fiber is one of the determinants of microbial species diversity.
