By phase-contrast microscopy, SC clones were easily distinguished on the basis of their proliferation rate into LPC (Fig. 1c, left) and HPC (Fig. 1c, middle), similarly to what observed in single fiber cultures.
We placed the fiber cultures into a Zeiss Environmental incubation system (5% humidity, 95% O2) at 22°C.
In vivo activity of VLX40 was investigated in hollow fiber cultures of myeloid U-937 cells subcutaneously implanted in mice.
To address this, we used primary single-fiber cultures in which myofibers are cultured with their associated satellite cells.
In 48 h, primary single-fiber cultures P-Fra-2 is expressed in Pax7-positive satellite cells, but myogenin expression does not appear until 72 h in culture and is not co-expressed with P-Fra-2.
Rat mesenchymal stromal cells (MSCs) were seeded on either PET or PU fiber-layered culture plates coated with laminin, collagen I, fibronectin, poly-d-lysine or gelatin.
The EPS-triggered changes of these genes in muscle fibers in culture were then compared to those in acutely exercised and trained mice, respectively.
The present work aimed to analyze alterations in lung small cell carcinoma cultures after 48 h of chrysotile exposure, followed by 2, 4 and 8 days of recovery in fiber-free culture medium.
Also, alterations in cell morphology were related to the presence of fibers in culture during the first 24 h and 48 h of chrysotile treatment and also after long periods of recovery.
Chrysotile treatments were done using three different fibers concentrations (250 µg/ml, 125 µg/ml, 62.5 µg/ml), and after 48 h of treatment were used three different times of recovery in fiber-free culture medium: 2, 4 and 8 days.
