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Loading and de-esterification buffers were supplemented with 100 μM N-benzyl-p-toluenesulfonamide (BTS Medchem Expressss, Lucerna-Chem, Luzern, Switzerland) to prevent fiber contraction and detachment.
Third, the physics of fiber contraction (or gels as they are more generally called) have become well understood (Tanaka 1973a - 87b).
Accordingly, the objectives of this study are to establish a baseline characterization of cardiac strains throughout the cardiac cycle, to quantify temporal, regional, and transmural variations of active fiber contraction, and to identify pathways of mechanical activation in the healthy beating heart.
While the metabolic gene expression determines substrate usage, other muscle fiber parameters, such as fiber contraction speed and peak force generation, are controlled by myofibrillar proteins.
In brief, depolarizing command pulses of variable duration (from 500 to 5 ms at 0.3 Hz) were progressively increased in amplitude from the holding potential (H) of −90 mV until a visible fiber contraction.
The workstation to test permeabilized fiber contraction has been described previously [11], and employs an AE801 force transducer (SensoNor, Norway) opposite a model 312C length controller (0.25 ms length step response time; Aurora Scientific, Canada).
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The first muscle fiber contractions occur just minutes after primary motor neurons emerge in a given somite, and large trunk flexions occur while somitogenesis is still ongoing (Myers et al., 1986).
In a small study of mechanically ventilated critically ill patients, passive movement of the leg attenuated loss of specific force (but not atrophy) measured by single-fiber contraction [ 101].
Fiber mat contraction drove expulsion of a glycerol/water solution stored in the pores of the mat, as evidenced by microscopic observations.
What then follows is a cycle of adhesion to the ECM, stress fiber formation, contraction, and detachment at the rear end of the cell [ 32].
The hysteresis loops models considering the effect of fibers Poisson contraction and effective coefficient of the fiber volume fraction in the loading direction were used to predict hysteresis loops corresponding to different fiber preforms, peak stresses and test conditions.
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