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The FIASCO procedure uses only MseI.
The fast isolation by amplified fragment length polymorphism of sequences containing repeats (FIASCO) procedure described by Zane et al. was used to isolate the second library (referred to as the KU library) at the Plant Breeding Laboratory Kyushu Universityy).
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The numbers of identified SSRs varied across these studies from almost all (97%) sequences with microsatellites (FIASCO enrichment procedure) [ 17] to several hundred (single lane of transcriptome sequencing) [ 33], with most studies falling somewhere in between.
If there is anything to be learned from baseball's steroids fiasco it's that the major sports leagues need to start regulating the use of this procedure, and be quick about it.
Last year, as the robo-signing fiasco forced its competitors to make embarrassing admissions or halt home seizures, Wells Fargo resisted, arguing that its procedures were sound.
Fiasco naturally ensues.
What a fiasco!
It was a complete fiasco.
"The pug fiasco," Shay said.
For Fiasco, the territory beckoned.
Project LQ was a fiasco.
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