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In contrast, the lungs and spleen did not show a pronounced maximum in total transcriptional regulation, and moreover, only few transcripts were regulated in both tissues.
In crambe, only a few transcripts such as cysteine proteinase, thioglucosidase, aldose epimerase, and PMSR showed increased transcription within 24 hrs of phosphate deficiency.
In both lung and spleen, few transcripts were consistently downregulated.
This argument could explain why few transcripts and biological processes were affected in two or more tissue types after irradiation.
In general, most regulated transcripts were specific for the different dose levels where few transcripts were affected at more than one dose level.
The majority of the affected transcripts were specific for the different absorbed doses delivered, and few transcripts were shared between the different tissues investigated.
Although only few transcripts were regulated in the lungs and spleen (Fig. 2), the associated cellular function differed distinctly through the day.
The response of the transcripts affected at all dose levels was generally found to be independent of dose, and only a few transcripts showed increasing or decreasing regulation with increasing absorbed dose.
For a few transcripts, riboswitches and small RNAs were found.
Very few transcripts (5) were distinctively overexpressed in the more differentiated population (Sca-1Lo-only cluster).
Commonly, microarray data normalization methods assume that relatively few transcripts change from sample to sample [36].
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