Second, when there is strong linkage disequilibrium (LD) in a population there are relatively few segregating chromosome units and then random segregation will have a marked influence on the variation in genome-wide heterozygosity also within each level of inbreeding [11].
Detecting population demographic size changes can be difficult with small sample sizes, few segregating sites or haplotypes, or when the population has experienced a very recent expansion [41].
Values of ρ were estimated only for loci with S ≥ 5, due to the poor performance of estimators when there are few segregating sites [44].
This is simply because genomes are very large and adaptive evolution probably occurs only at a few segregating mutations at a given time [ 2].
Very few segregating SNPs were shared between EB and PB (1.44%) and EB and WB (1.89%) when compared to the overlap between PB and WB (41.91%).
Additionally, this observation suggests that the pre-exposure expression differences of many genes, rather than only a few, segregate the KL cells of t-AML susceptible versus resistant strains.
Tajima's test, on the other hand, was not significant for these sequences, although the fact of being four sequences with few segregating sites along the alignment might influence the ability of the test to detect selective pressures operating on them (Additional File 5).
Therefore, we expect fewer segregating sites observed in regions under recent positive selection.
This is not observed for the analogous case with larger deleterious effects (s = 0.5, h = 0, L = 1), probably because there are fewer segregating deleterious alleles and linkage between them is therefore weaker.
