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CHIKV 06.21 replicated at a high level and homogenously whereas CHIKV 05.115 showed two distinct profiles: most of females ensured a low replication level and only a few replicated CHIKV 05.115 as efficiently as CHIKV 06.21.
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Although these data are not surprising based on the relatively high dose rates, they are important to future work at the LoDIF because they confirm the utility of our chosen model organism for detecting population-level responses, and they illustrate the statistical power achieved from using replicated mesocosms, in that statistical significance was achieved with few replicates per treatment.
In a NOEC analysis involving only few replicates, the detection of a small effect cannot be expected due to the small statistical power of a statistical test on binary effects with few replicates [14].
Very few replicate studies have re-evaluated the prevalence of primary headaches in this age range and it is uncertain whether the prevalence is increasing.
As the macroinvertebrate samples consist of only few replicates I followed recent developments in statistical reasoning and calculated the confidence interval (CI) of the difference that ozonation makes [28, 29].
As a NOEC is typically reported without the circumstances of its genesis, a user cannot comprehend whether a high NOEC is either due to weak toxicity or to an experiment with few replicates [15].
With few replicates and high variability both within and among these large-scale treatments, investigators face the possibility that differences might only be detectable at untraditionally high significance levels.
Elasticity analysis indicated that adult survival, and litter size of three- and four-year-old females are most important to λ in 99.3 and 0.7% of all replicates, respectively; the few replicates with higher elasticity for litter size occurred in scenarios with some type of correlation among vital rates; of these, 21 (70.0%) occurred in scenarios with 20% reductions in subadult and adult survival.
In addition, we used a conservative burn-in value of 1,000 (manual verification of a few replicates indicates that the burn-in is less than 500).
The discrepancy may be a consequence of the use of early filter-based hybridization technology with few replicates or differences in environmental conditions and genetic background between the studies.
However, there are usually only a few replicates (usually three) within each group.
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