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The main limit of the study is due to the retrospective nature of the analysis: in fact, only few data, not planned in a specific well defined protocol with timing, are available in the clinical databank, even if there was the authorization of the local ethics committee for tissue samples at the transplantation time.
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If other more costly NS are used, SCIT therapy becomes increasingly more cost-effective in comparison and the number of years between rounds of shots becomes fewer (data not shown).
During the initial stages of SCX, buffer blanks were run after each sample, and the resulting fractions were assessed for peptide carryover; apart from peptides corresponding to a few high abundance proteins, we found very few peptides (data not shown).
Throughout evolution, the networks acquired more realistic properties, such as having a few nodes with many outgoing connections (hubs) and many more nodes with very few connections (data not shown).
Thus in our procedure from time to time new clusters will be formed, composed only by a few data points, not sufficient to obtain a positive definite estimate of the corresponding covariance matrix using the classical sample covariance estimator.
During this analysis, a few data points not routinely recorded on the database were found to be important and were retrospectively compiled from the paper-based medical record.
Since the real-time PCR technique is known for producing linear response over a wide range of starting concentrations [ 6], and these few data are not too distant from the extreme values used to produce the standard curves, we considered them acceptable.
In these studies, plasma was analysed for cytokines by ELISA, but as expected, IL-4 was detected in very few samples (data not shown).
Amputation was not assessed due to very few events (data not shown).
The bandings for the different age groups were very similar with few differences (data not shown).
Larger extensions existed, albeit with barely more than a few transcripts (data not shown).
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CEO of Professional Science Editing for Scientists @ prosciediting.com