During 30 months of field trial (2010 2012) in ICAR regional station, Lembucherra, West Tripura, the control plot (T 0) received no fertilizer, while experimental plots were fertilized with four different amounts of vermicompost viz.
Soil CO2 emissions were measured during the growing seasons of 2003 and 2004 from plots fertilized with three N rates (0, 135, and 270 kg N ha−1) in continuous corn and a corn soybean rotation in a split-plot design.
Although litters were obtained from mice fertilized with all three microinjections, only those from BAC2 and BAC5 transmitted the germline gene to their offspring (Fig. 1A and data not shown), and only those from BAC2 exhibited expression of the huCEACAM1 protein in their feces (Fig. 1B).
More specifically, experimental plantations were established in nine fallow fields, each of which was divided into four plots fertilized with one of four levels of hog manure supply (i.e. 0, 40, 60 or 80 kg ha−1 in total N).
However, in cases of high fecundity, egg lots were split and fertilized with more than one male to create more families.
A significant (P<0.01) reduction in the overall number of fertilized oocytes with two well formed male and female pronuclei was observed in the treatment group compared to the control group (58.5±5.8% versus 73.3±3.6%, respectively).
Oocytes were examined for the presence of pronuclei and polar bodies 18 20 h post insemination (p.i .. Normally fertilized eggs with two pronuclei and two polar bodies were either cultured under oil in 30 µl drops of medium (Medicult Universal Medium until May 2005, Cook's Cleavage Medium thereafter) at 37°C in an atmosphere of 5 6% CO2 in air, or frozen immediately.
The oocytes were fertilized with frozen semen from two different AI bulls with proven field fertility, one of the SR breed and one of the SH breed.
Bermudagrass growing on a poultry layer litter amended site was fertilized with ammonium nitrate at four rates (0, 56, 112, and 168 kg N ha−1) on 28 April and 19 July 2000, then harvested on 10 May and 18 August 2000.
