From these data we calculated the parental fertilization proportion as the proportion of sexual females producing diapausing eggs.
The parental fertilization proportion was similar for both types of crosses, suggesting that there is no mechanism to avoid selfing.
On the other hand, our results suggest lack of selfing avoidance in B. plicatilis, since the parental fertilization proportion of selfed and outcrossed crosses did not differ.
For each population, we compared both the parental fertilization proportion and F1 fitness components from intraclonal (selfed) and interclonal (outcrossed) crosses.
Additionally to the F1 fitness components, we compared the parental fertilization proportion –i.e. the proportion of sexual females being diapausing egg-producers– between both types of crosses, in order to explore if there were signs of inbreeding avoidance.
Although P-clone combinations had a significant interaction effect on the parental fertilization proportion, the most negative P-clone interaction coefficients did not tend to occur in selfed crosses (Fig. 2, PFP).
Moreover, the global parental fertilization proportion was similar for selfed and outcrossed crosses in both TOS (Poza Sur, the smallest population; see Methods for details) and HOS (Hondo Sur) populations (Fig. 3).
Measurements performed for both the clonal crosses and the F1 generation resulted in estimations of six response parameters: the parental fertilization proportion, the F1 egg hatching proportion, the F1 clone viability, the initial net growth rate R, the proportion of male-producing clones and the proportion of diapausing egg-producing clones.
Two studies on adult fish, Pimephales promelas, found no significant effect of atrazine on number of eggs produced, fertilization success, proportion of hatchlings, or larval development.
