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Our results also contrast with those of a recent study with a similar design to ours, in which grasshoppers in long term fertilization plots in an N-limited prairie did not increase in abundance with either N or P addition, although fertilization did alter leaf stoichiometry and diet composition [30].
Our research area was created by the 1980 eruption of Mount St. Helens (46°15'N, 122°10'W), and is located on primary successional pyroclastic deposits and on debris flow deposits on the volcano's north slope (1050 1170 m) (See "Fertilization plots" in Fig. 1 of Gill et al. (2006) for additional site description).
Samples were taken on each harvesting date from both 0 and 60 kg/ha fertilization plots.
Our first hypothesis was that fertilization of switchgrass increases root biomass, SOC, and total N pools relative to non-fertilized plots.
In Ohio, 4 years of N fertilization of switchgrass at 202 kg N ha−1 reduced the amount of macro-aggregates at the 0- to 5-cm depth compared with non-fertilized plots [22].
The split-plot scheme was used, with four fertilization treatments in plot and sub-plot evaluation times.
The experiment comprised 36 treatment plots arranged in a split-plot design, with N fertilization on major plots (either 70 or 100 kg N ha−1 applied at sowing) and water regime on sub-plots (either limited water supply, natural (rain-fed) water supply, or natural water supply + irrigation).
Although decreased pH had an overall negative effect on percentage fertilization, the scatter plot revealed that two pairs actually showed a positive response in decreased pH conditions with an increase of 36%and16%6% compared to the control, also seen for Sterechinus neumeyeri and Centrostephanus rodgersii in response to acidification scenarios (Foo et al. 2012; Sewell et al. 2014).
This study was conducted in a split-plot design, with fertilization as a main plot and compaction as a subplot.
This experiment was established in a split-plot design with fertilization as a main plot and soil compaction as a subplot.
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