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The understanding of this very complex process could be implicated among in vitro fertilization methodology and regenerative medicine.
In vitro fertilization methodology is sufficiently advanced for gametes of several mammalian species, especially mouse, rabbit, and rat, for use in evaluating effects imposed by toxic agents of environmental or genetic origin.
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Consulting relevant literature and collaborating with colleagues from other disciplines may provide important opportunities for cross-fertilization in developing improved methodology and measurement.
Using central composite design and response surface methodology, the experiment of fertilization and hatching success in Pinctada martensii (phylum Mollusca, class Bivalvia, order Pterioida, family Pteriidae) was undertaken under laboratory conditions.
11 The science and methodology of in vitro fertilization (IVF) have improved considerably in the last 25 years, offering the obvious benefit of boosting the odds of getting pregnant, sometimes from almost zero.
The need for definitive characterization through utilization of appropriate methodology derived from cross-fertilization of related disciplines is stressed.
The comparison of different anesthetic methodologies for sedation during in vitro fertilization procedures (EMLA cream, propofol, thiopental sodium, and sevoflurane) showed no differences in pregnancy rates [ 11].
Finally, the methodology accounts only for carbon sequestered directly by the plantations, corrected for climate change and CO2 fertilization (i.e. indirect human influences).
The combined effects of temperature and ammonia concentration on the percent fertilization and percent hatching in Crassostrea ariakensis were examined under laboratory conditions using the central composite design and response surface methodology.
Fertilization is external.
In vitro fertilization (I.V.F).
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