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It would have been more convincing if they had recovered a fertile double homozygote but if the PCR data are compelling, then they likely have identified complemented individuals.
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The plants with seed set were scored as fertile doubled haploid plants as these determine the success of the procedure.
The rate of fertile doubled haploid plants from the colchicine treatment at the seedling stage was 52.3% (Table 2), while the percentage of fertile plants in the negative controls, which is equivalent to the spontaneous doubling rate, averaged 32.1%.
While Dkk1 heterozygous mice are normal and fertile, mice double heterozygous for Dkk1 and the BMP inhibitor Noggin show head defects similar to those of Dkk1 homozygous mutants (del Barco Barrantes et al., 2003).
In Arabidopsis, although both single mutants of pksa/lap6 and pksb/lap5 were fertile, their double mutants had a male sterile phenotype (Dobritsa et al. 2010; Kim et al. 2010).
It had such a fertile field for double meanings of words, and just different ways of looking at it".
Six Saccharomyces species were used in the study, in which sterile hybrids of S. cerevisiae and five closely related species were made fertile by genome doubling and achieving tetraploidy.
In sharp contrast, P5CS expression was essential for pollen fertility, and p5cs1/ p5cs2 double mutant ovaries had a reduced success rate to develop fertile seeds even when fertilised by wildtype pollen.
The in vitro colchicine method tested here (Table 2) resulted in improved frequency, with the best treatments more than doubling the rate of fertile plants as compared to the spontaneous doubled haploid rate.
In addition, Rev-Erbα−/−Per1Brdm1 double mutants were fertile and gave birth to normally sized litters at the expected intervals (Fig. S2B, S2C).
A previous study reported daf-2 e1370); daf-2 e1370) isp-1 qm130 isp-1 qm130tile adoublewith a lifespan extension roughly additive of the two single mutants' [ 10].
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