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The EDA was added gradually into the ferric solution.
The solution was added dropwisely into the ferric solution.
After the addition of flower extract to ferric solution, a black-colored colloidal solution was formed.
Next, three different mineralizing agents were added into the ferric solution.
Fig. 1 The UV-vis absorption spectra of flower extract, ferric solution, and ZVIN solution.
In the former stage (chemical step) H2S is absorbed in a ferric solution.
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In our experiment, magnetite nanoparticles were synthesized by co-precipitation of ferrous and ferric solutions with a molar ratio of Fe(III)/Fe(II) of 2 1 at 80°C [27].
The desired physical and chemical properties of magnetite nanoparticles are synthesized by several chemical synthetic routes like co-precipitation of aqueous ferrous and ferric solutions [19], microemulsion technique [20] and hydrothermal synthesis [21].
SEM images of iron oxide particles prepared with the addition of (a) 5 ml of 10.67 M KOH, (b) 1 ml of EDA, and (c) both 5 ml of 10.67 M KOH and 1 ml of EDA into the ferric solutions.
The chromogenic reagent was prepared just before use by adding 5 mg of thiosemicarbazide to 50 mL of diacetyl monoxime solution (500 mg of diacetyl monoxime dissolved in 100 mL of distilled water) and mixing in 100 mL of acid-ferric solution.
Then, acid-ferric solution (containing 15% H2SO4, 60%H3PO44 and 0.005% FeCl3) and DAM-TSC solution (containing 1% diacetyl monoxime and 0.06% thiosemicarbazide) were added to stop the reaction and the mixture was heated at 100°C for 10 min for coloration.
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