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Once the glucose was exhausted, which occurred approximately at 12 h of the initial batch fermentation, the second phase of the fermentation was started with fed-batch mode.
Upon cultivating this E. coli strain in pure minimal media, the acetate concentration did not exceed values of 0.35 g L−1, even when the batch fermentation was started with a glucose concentration of 130 g L−1.
Fermentation was started by transfer of a piece of overgrown PDA agar to the fermentation media.
Subsequently, the medium was circulated again, and the next batch fermentation was started with the biofilm on the fibrous matrix.
The fermentation was started with an eight-hour batch phase, using 438 mL WL liquid (130.2 g/L glucose).
M-media was supplemented with 30 gDM WS/L, and the fermentation was started by transfer of 200 mL of the pooled pre-cultures into the bioreactor.
Similar(50)
Fermentations were started with different precultures and were performed in triplicate with the given standard deviations.
The fermentations were started by adding 400 mL preculture grown on YPD medium in 1-L baffled shaking flasks at 125 rpm and 30°C overnight.
The fermentations were started by adding 25 mL of preculture grown on YPD medium in 250-mL baffled shake flasks at 125 rpm and 30°C overnight.
The fermentations were started at a cell density of 1.3 g/L, in 60 mL volume at 30°C with continuous stirring at 200 rpm.
The batch fermentations were started by growing cells on 30 g of glucose in an initial working volume of 300 mL.
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