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The association between cross-tolerance to multiple stresses and fermentation outcome, as well as fermentative growth and stress tolerance was examined.
Autophagy is critical for fermentation outcome in primary fermentation as shown in our study, with 13 related genes identified as FEGs.
Fermentations conducted at this scale allow greater control over parameters which influence experimental reproducibility and fermentation outcome, such as inoculum preparation, maintenance of anaerobiosis and the inclusion of biological triplicates.
The relevance of Vam3p to fermentation outcome and tolerance of stress (hyperosmolarity, heat, ethanol and oxidation) is highlighted by the protracted fermentation of the Δvam3 mutant (Additional file 1) and growth sensitivity towards these stress parameters (Additional file 2).
Hydrogen generated from the applied current shifted the fermentation outcome from predominantly C2 and C3 VFA (64 ± 3%% of the total VFA present in the control) to majority of C4 to C6 (70 ± 12%% in the experiment), with identical proportions in the VFA acid extract.
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In order to optimize the fermentation outcomes, fed-batch fermentation was performed with hydrolyzed molasses in PFB, giving 91.89 ± 4.59 g l 1 of propionic acid at 254 h.
However, the effects on fermentation outcomes were not elucidated.
Higher amounts of hydrolysate in the propagation feed further improved the fermentation but increased the variability in fermentation outcomes and resulted in up to 20%% loss of cell mass yield.
The tight regulation of these genes is of paramount importance for the fermentation progress and outcome [ 45], in particular if one considers that arginine and proline are the most abundant nitrogen sources in grapes [ 65].
In the wine making process, thiamine levels play a critical role in the outcome of fermentation and a lack of thiamine causes sluggish or stuck fermentation [ 3, 4].
Increased variability in cultivation outcome and fermentation was also seen with higher amounts of inhibitors in the pre-adaptation process, which constitutes a technological risk.
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