Solid state fermentation or cultivation (SSF) involves the use of a culture medium composed of solid material with given moisture content.
Results from this study showed that the application of fed-batch fermentation in cultivation of P. acidilactici has significantly reduced the accumulation of lactic acid in the culture with significant increased in the viable cell number.
Solid-state fermentation is a cultivation technique in which microorganisms are cultivated on moist solid particles, in beds within which there is a continuous gas phase between the particles.
Subsequently, the liquid seed was transferred into a 100-L fermentation tank for cultivation under identical conditions for 20 d to afford an 80-L culture broth.
The "Minimal medium" composition as mentioned in section 'Fermentation medium and cultivation' was used, and yeast cells of 16 g/l were added in the fermenter in the case of free cells cultivation.
For fermentation experiments, seeds cultivation was conducted in 250 ml shake flasks containing 30 ml fresh seed culture for 12 h and then inoculated (5%, v/v) into a 250 ml shake flask with 50 ml of fermentation medium at 30 °C on a rotary shaker at 200 rpm.
Yet most of the natural products are still supplied primarily through fungal fermentation and plant cultivation accompanied by subtle chemical modifications.
The main contributors to the total direct cost were the pretreatment unit, the fermentation stage (yeast cultivation and SSF) and the CHP plant.
Next, 5% seed culture (2.5 ml) was inoculated in a 250-ml conical flask containing 47.5 ml of fermentation medium, and cultivation was carried out in a rotary shaker at 25°C and 160 rpm for seven days.
Unfortunately, even though well-characterized microorganisms were used in the fermentation system, a cultivation of two different organisms in the same bioreactor was still difficult because of the differences in their metabolic types, optimal conditions, and nutritional requirements.
Subsequently, the liquid seed was transferred into a 100 L-fermentation tank for cultivation under the same conditions for 20 days to afford an 80 L culture broth.
