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These results were in close agreement with models of FEN substrate interaction previously proposed from site-directed mutagenesis and substrate-binding studies [ 25, 26].
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FENs recognize substrates with a DNA duplex and a single-stranded 5′ overhang such as might arise during strand displacement synthesis.
The placed peat serves two major functions of attenuating the upwelling of dissolved solutes from the underlying tailing sediments to the rooting zone, and as fen vegetation establishment substrate.
Dominant vegetation and other substrates used in fen construction, like peat, petroleum coke, tailings sand and upland soil, were collected for the study.
The first co-crystal structure of a FEN in complex with a bifurcated substrate has recently been solved [ 24].
Sedges and trees produced the largest amount of DOC, followed by peat and moss in all the fens, whereas the substrates from the constructed fen such as petroleum coke, tailings sand and upland soil produced very little DOC per unit dry mass.
These results suggest that revegetation strategies and water chemistry might be more important controls on GHG fluxes than substrate quality in a constructed fen.
However, the GHG fluxes of a fen that is constructed with drained peat substrate and receives runoff containing effluents of salt and naphthenic acids is not known.
DNA genetic integrity and cancer avoidance depends upon the structure-specific repair and replication nuclease flap endonuclease (FEN-1) and upon the trimeric processivity factor PCNA. FEN-1 and PCNA complex structures and mutational results provide a coherent model for DNA substrate recognition and PCNA activation of FEN-1.
We investigated DOC production and its bioavailability from substrates collected from a constructed fen and natural fens (reference ecosystems) in the Athabasca oil sands region near Fort McMurray, Alberta.
On bifurcated substrates, SaFEN exhibited similar substrate specificity to previously characterized FENs.
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