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The present study was designed to determine the age and female specificity of a membrane protein that binds to a pheromone biosynthesis activating neuropeptide (PBAN) ligand and to elucidate the effect of Juvenile Hormone (JH) on binding as well as pheromone activation.
Additional file 2: Table S1 summarises transcript name, length, best BLASTx hit, predicted domains, and male or female specificity.
This increased to 174 genes by 4 wk, with two thirds of these genes showing female specificity.
In the present study, we investigate sequence polymorphism in the female specificity determinant SRK of Arabidopsis halleri from throughout Europe.
This is the first report of sequence polymorphism at the female specificity determinant gene SRK in the self-incompatible Arabidopsis halleri.
Since PiSBP1 interacts with both male and female specificity determinants, it may play a key role in S-RNase-based SI.
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For females, specificity was poorest (specificity = 0.45 to 0.55), however sensitivity was perfect (sensitivity = 1.0) (Table S2 in Additional file 2).
Thus TSE can occur in third instar larvae and presents the same maternal effect and female germline specificity as in adults.
In contrast, the G12 upstream region was able to faithfully reproduce the expression profile of the endogenous A. gambiae gene, showing female midgut specificity in the adult mosquito and massive induction PBM, peaking at 24 hours.
RT-PCR confirms the female SG specificity of these transcripts.
In this light the bloodmeal inducibility and strict female midgut-specificity imparted by the pG12 construct give it a potential advantage over constructs containing promoter regions from other bloodmeal-inducible A. gambiae genes such as AgCP and Aper1, which show some constitutive activity in the male gut or the female gut or both[2], or the Antryp1 studied here.
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