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All recorded test feeds were performed by the duty surgical registrar.
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Gross energy of feeds was performed on dried samples by adiabatic bomb calorimetry.
RNAi by feeding was performed as previously described [70], [71].
RNAi by "feeding" was performed essentially as described by [20], ("Protocol I").
RNA-Mediated Interference Clones RNAi by feeding was performed as previously described (Kamath et al., 2001 ).
Analysis of feeding was performed by cultivating animals on GFP-labeled E. coli bacteria.
RNAi by feeding was performed as previously described (Kamath et al., 2001).
RNAi by feeding was performed as previously described [ 11] with the following modifications.
RNAi by feeding was performed as described using 6 mM IPTG (50).
EdU labeling by feeding was performed similar to previous studies (Crittenden et al., 2006; Fox et al., 2011; Morgan et al., 2010).
RNAi by feeding was performed at 20° according to a standard protocol, starting with L4 larvae moved every 12 hr to fresh RNAi plates (Timmins and Fire 1998; K amath et al. 2001).
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