For glucosinolate analyses, the upper 2 cm of the main root, where root fly larvae are typically feeding, were collected and frozen at −20 °C.
During pregnancy and at 3 and 6 months postpartum, data on maternal and infant socio-demographic, behavior, and feeding were collected.
While the acquisition access period (AAP) is likely a little shorter than the time point collected, only individuals in the process of feeding were collected.
The mice were finally sacrificed, and fed serum were collected and subjected to the measurement of triglycerides (TG), non-esterified free fatty acid (NEFA), total cholesterol (TC), and high density lipoprotein (HDL).
Treatment periods were 21 d; milk and feed samples were collected and milk yield and feed intake were recorded for the last 4 d of each period.
During the study period, from April , 2012to May, 2013 fish feed ingredients were collected and then transported to the Zoology Laboratory, Visva-Bharati, Birbhum, W.B, India (23°41′30″N Latitude and 87°41″20E Longitude).
To corroborate the changes in the oxidative burst in the NILs in response to D. noxia biotype SA1 feeding, leaves were collected and stained for H2O2 using 3,3′-diaminobenzidine (DAB), which forms reddish-brown polymerized deposits in the presence of peroxidase (Fig. 6).
Spinach plants inoculated with the Tat-producing constructs were collected and fed to mice 7 14 days post inoculation.
Newly emerging males were collected and fed for 2 days on defined food media.
