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Experiments on real conditions by using real feeds were carried out in the absence of reaction.
Feeding strategies aimed at improving the nutritive value of crop residues through chemical treatment or utilizing other available feed resources as small ruminant feeds were carried out during the hot dry summer season when rangeland pasture deteriorates drastically.
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Continuous 2% peptone and 1% yeast extract feeding were carried out for about 6 h in the subsequent fed-batch phase.
The posterior leaflike gills serve principally for respiration; feeding is carried out by the palp proboscides, which collect surface detritus.
RNAi by feeding was carried out as described by (Kamath and Ahringer, 2003).
Further inspection, to identify peptides potentially involved in feeding was carried out with user-defined scripts in R applying two strategies.
Labeling of tick β-actin dsRNA for tracking dsRNA during feeding was carried out using fluorescent labeling of dsRNA with Cy™3 Silencer siRNA labeling kit (Ambion) with minor modifications to the manufacturer's protocol.
The non-synchronous feeding was carried out in a non-stoichiometric way, where 1.0 mL of solution B was transferred to a reactor and the remaining 9 mL of solution B and 10 mL of solution A were then fed synchronously (1 mL·h−1) into the reactor under rigorous stirring at RT.
Feeding is carried out by first shifting the mandrills to their off-exhibit enclosure while the food is scattered around the exhibit enclosure, after which the mandrills are re-released.
Feeding was carried out for one hr using Hemotek membrane feeders (Discovery Workshops) heated to 37°C.
Animal handling, control and special feeding was carried out by MMR.
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