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Extrusion of feeds was done using a small-scale single screw extruder at Bidii Fish Farmers, Luanda-Vihiga County, Kenya.
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Multiple feeds are done using the same parasite-source, i.e. blood from the same volunteer, to test different titers and different antibodies.
Feeding was done 5 times a day throughout the study period.
The phage biocontrol was null even two months passed since last time phage feeding was done [data not shown].
Feeding was done in lots of 20 mL FS every 5 hours and was automated via the DASGIP bioreactor's pump module.
Feeding was done using defibrinated calf blood (Colorado Serum Company, Denver, CO) containing 11.75 ng/ml IVM (10 mg/ml stock solubilized in dimethyl sulfoxide (DMSO) and diluted in phosphate buffered saline (PBS)).
The third method of phage master mix feeding was done using absorbable vegetable capsules (ZeinPharma Germany GmbH, Germany) made of Hydroxypropyl Methylcellulose (HPMC) administered orally into the tested rats.
Experimental feeding was done for a period of 90 days.
Experimental feeding was done for a period of 150 days including a 6 days metabolism trial.
The diets were fed ad libitum twice daily, and the control of feed was done individually, by daily weighing of food provided and remains, allowing between 5 and 10% of orts.
One would get up and need feeding, and when that feeding was done, the other would start fussing to be fed, so it worked out.
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