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During the acclimatization and whole experimental period no feeding was administered to the trilobite larvae to avoid any potential increases in metabolic (and thus respiratory) rates associated with specific dynamic action.
Feeding regimen of the VLBW infants was not changed during the study period: enteral feeding was started at day 1 or 2 and complementary parenteral feeding was administered until enteral intake reached 100 ml/kg/day.
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Vaccine-treated feed was administered twice a day for 1 week, and priming and boosting were performed with a 1-week interval in between.
Feed was administered in two equal portions of 2.5% of the mean body weight at each feeding.
Feeds were administered by pouring into a sterile syringe (without plunger) that was attached to the tube, and allowed to flow into the stomach by gravity.
Supplements (one daily oral dose mixed in the morning feed), were administered in spaced doses, 7 days before arrived to the schooling area and every day until the end.
In patients receiving intragastric feeds insulin was administered if blood glucose concentrations were >15 mmol/l [ 12], whereas during post-pyloric nutrient delivery insulin was administered as per unit protocol (>10 mmol/l) [ 13].
In the domperidone group, domperidone (20 mg tube feeding × 4/day) was administered by the tube immediately after it was successfully placed into the stomach.
Postoperatively, immediate feeding (regular food) was administered, no further antibiotics were given.
Doxycycline was administered by feeding mice with dox-impregnanted food pellets (625 ppm; Harlan-Teklad).
Doxycycline was administered by feeding mice with doxycycline-impregnated food pellets (625 ppm, Harlan-Teklad).
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