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DOI: http://dx.doi.org/10.7554/eLife.06104.001 Throughout the life of a female mammal, the mammary glands undergo different phases of development to prepare for, and adapt to, feeding offspring.
Although regurgitation of blood for feeding offspring or roost mates was suggested to be a route of infection in vampire bats (8 ), the organ distribution of RABV in our study suggests secretion from salivary glands after spread from the central nervous system, which is compatible with virus transmission in other mammals.
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Maternal undernutrition resulted in a significant reduction in weaning weights of chow-fed and HF- fed offspring of all undernourished groups (p<0.001) (Table 2).
Post-hoc analysis demonstrated a statistically significant increase in AG % BW in HF fed offspring of UNP and UNPL mothers (p<0.001 for both).
The significant benefits of breast milk are evident through a reduced risk of infection, asthma, and development of breast cancer in breast fed offspring [3] [5].
Post-hoc analysis demonstrated a statistically significant decrease in chow-fed and HF- fed offspring of mothers undernourished throughout pregnancy and lactation (UNPL, p<0.001; UNPL-HF, p<0.001) compared to chow-fed and HF- fed Controls.
Furthermore, hepatic expression of let-7c was also reduced in maternal HF fed offspring.
But in our control fed offspring, where disease is not yet present, we find that metabolic genes are practically absent from comparisons between lean- and obese-born mice.
Having observed reduced expression of let-7s in maternal HF fed offspring, we measured the global miRNA expression profile with microarrays.
The mRNA level of Igf2 was increased by ~2.7-fold in maternal HF diet fed offspring compared to the control animals (p < 0.01, Table 1).
Levels of miR-30c between maternal HF and chow fed offspring were similar when measured with microarrays, but significantly different when measured with qPCR.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com