However, the carp in our study were fed commercially available food with high protein and low plant content.
Mice –Virgin or lactating mice (lactation day 4 5) were obtained commercially (Charles River) and fed commercially available rodent chow ad libitum.
Our own experience with captive eastern moles, which we feed commercially available nightcrawlers (Lumbricus), indicates they may easily consume their body weight in worms each day.
The animals were fed commercially available pig feed and kept on a bedding of wood shavings, which is considered less likely to harbour mycobacteria, as opposed to sawdust [ 11].
Teaching to care giver was given regarding the care of the PEG tube and feeding; using commercially based nutritional feeds, along with home based blenderized diets.
In addition to that, information on the nutritional requirement of O. marmorata is still limited up-to-date (Cheah et al. 1994; Lin and Kaewpaitoon 2000; Yong et al. 2015), and there is still no compounded feed commercially available for this fish.
Animals were fed with commercially available standard pellet diet (VRK Nutrition Solutions, Sangli, Maharashtra, India Ltd .. Drinking water was supplied to the animals, ad libitum.
Fish used for cell culture were maintained in 1 m tanks at 10.8 C, and fed a commercially available fish diet (Ewos Innovation).
The animals were fed with commercially available feed and were maintained under standard conditions of temperature (25°C ± 5°C), relative humidity (55 ± 10%), and 12/12 h light/dark cycle.
Mice were housed in a conventional animal house facility with a 12 12-h 12 12-hark cycle, fed a commercially available diet, and given water ad libitum.
