Sentence examples for feeder conditions from inspiring English sources

In addition, these settings can be tuned according to the changes in the feeder conditions, e.g. topology and load amounts.

In line with these findings, FGF-iPSCs expressed Nanog, Rex1 and Stella (Dppa3) at similar levels to those detected when cultured on feeder conditions, and the EpiSC markers Cer1 and FGF5 were not found up-regulated as tested by qPCR (Fig. 7I).

Furthermore, porcine iPSCs from these conditions were placed into MEF feeder conditions, and there was no visible differentiation in addition to those that were maintained in the traditional feeder system and 1(i)).

hESC were allowed to grow in feeder-conditioned hESC medium for 24 hours or until 50 70% confluent.

For these two passages, TSCs were cultured in 70% feeder-conditioned media plus growth factors, as described in (Quinn et al. 2006).

Human ES cells H9 (Wicell, WI) and mouse W4 ES cells (kindly provided by the Gene Targeting Core Facility, University of Iowa) were cultured, either with feeders or in feeder-free conditions, as described previously [22], [13].

For feeder growth conditions, H9 (WiCell) hESCs were cultured and maintained according to the methods recommended by WiCell.

H9 hES cells were also cultured under feeder free conditions using MEF-CM [26] and mTeSR1(StemCell Technologies, Vancouver, BC, Canada) following the manufacturer's instructions.

At least three passages under feeder free conditions on 0.1% gelatin were used to exclude feeders.

To test for genomic integration and residual retention of episomal plasmids, each iPSC line was migrated to feeder free conditions and grown beyond passage 15 on hESC-qualified Matrigel (1 100 dilution, BD Sciences) coated plates in Essential 8 media (Life Technologies).

Embryonic day 14 (E14) mouse ES cells were either co-cultured with mouse primary embryonic fibroblast feeders or cultured under feeder-free conditions [ 23].