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Fish were fed once daily (9 00 10 00) to apparent satiation, and uneaten feed was collected to determine feed consumption.
Uneaten feed was collected after 1.5 h of feeding.
The uneaten feed was collected 30 min after feeding and then oven-dried at 70 °C.
Waste feed was collected 0.5 h after each meal in order to measure feed intake and appetite at tank level, and to calculate feed rations.
Feed intake was monitored throughout the trial and waste feed was collected from the effluent water from each tank by a wire mesh collector and dried.
Based on this history, information regarding dates corresponding to recent feed deliveries, the location of the associated storage bin, the period of time between delivery of feed and clinical signs, the location of index cases in each farm, mill source and whether porcine by-products were present in feed was collected during the investigation.
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Three batches of unmedicated feed were collected after one batch of medicated feed.
Once approximately 50 grams of feed were collected, a 10 mL aliquot of sterile saline was added to the specimen container.
During the rearing period of the calves, samples of milk formula concentrate and dehydrated fescue feed were collected to have a mean sample to determine DM, nitrogen (N), fat and minerals according to previously described methods [ 14].
The community-based approach and the fact that data on breast-feeding was collected before the onset of disease give the study strength.
Neither precise age at death (in months) nor a history of breast-feeding was collected for this study, so direct exposure to drinking water remains a consideration.
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