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concentration of metabolite m ex,j in feed media.
Usually the concentration of asparagine was only half of glutamine concentration in feed media.
Feed media (glucose) was added to introduce a carbon source and for the purpose of achieving higher cell density.
All the feed media had the same composition and were prepared as described above except for the carbon source.
Therefore, how to correctly supply asparagine and glutamine in feed media to achieve high mAb production becomes a critical issue.
Our research also demonstrates that MBRs can support relatively complex bacterial communities even on simple feed media.
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In fact, microbial contamination was not perceived during the cultures, even if the feeding media was not sterilized.
Macrophage-like cells and C. neoformans or beads were incubated together for 60 min to allow for completion of phagocytosis, washed once with fresh media, replenished with 2 ml feeding media and followed by time-lapse imaging every 4 minutes.
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