For scale-up production of PaE, under xylose feeding cultivation of P. antarctica using a jar fermentor (Watanabe et al. 2014b), we found a highly secreted 33-kDa unknown protein (unpublished data).
Results of the YQ4 strain pyruvate-feeding cultivation indicated that redistributing more carbon fixed by cyanobacteria to pyruvate might be useful strategy for enhancing ethanol productivities.
In this work, we provided a paradigm for systematically characterizing and optimizing the PDCzm-slr1192 pathway from engineered cyanobacteria strains, combining in vitro reconstitution, genetic engineering and feeding-cultivation.
In summary, we combined in vitro reconstitution, genetic engineering, and feeding-cultivation for systematically characterizing and optimizing the PDCzm-slr1192 pathways in engineered cyanobacteria strains.
In this work, we adopted a systematic strategy combining in vitro reconstitution, genetic engineering and feeding-cultivation to characterize and understand the PDCzm- slr1192 pathway in ethanol synthesis strains of Synechocystis sp. PCC6803 (Synechocystis hereafter).
In this work, we provided a paradigm for systematically characterizing and optimizing an essential ethanol synthesis metabolic pathway of engineered cyanobacteria strains, combining in vitro reconstitution, genetic engineering and feeding-cultivation.
Already, more than 80% of the world's soybeans are used as feed; their cultivation takes up huge amounts of land and water.
Under the dietary change scenarios, CO2e emissions fall reflecting an increased vegetable and fruit production and a decreased grassland farming and animal feed crop cultivation.
Another concern is the increased variance in viability with higher hydrolysate liquor content in the feed during cultivation.
After complete consumption of glycerol in the batch phase, fed batch cultivation was initiated by starting the balance controlled feed pumps.
The model equations were extrapolated to fed-batch cultivation by incorporating the dilution terms due to fresh nutrient (carbon and nitrogen) feeding.
