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Triglycerides were collected in the fasted state (65%) or fed state (18%), with 17% having an unknown fed status.
Drug administration in the present study was conducted with subjects in fed state.
In vivo studies presented in Table 1 were performed either in fasting or fed state.
The truth is, being in a consistently fed state is not natural to the body's physiology.
It is also established that amino acids can fuel gluconeogenesis when delivered in abundance even in the fed state [27].
The second explanation is that the shift is due to the change from a starved to a fed state.
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Furthermore, we fasted mice in all groups overnight to determine body weight in the non-fed state.
In the well-fed state, excess citrate is withdrawn from the TCA cycle and transported out of mitochondria.
Moreover, plasma insulin levels in the re-fed state tended to be lower in LMKO mice compared with controls.
Cuffdiff was applied to compare gene expression of LMKO to that of control under fasting and re-fed state.
PCr/ATP was 32% lower in the fasted state compared with the glucose-fed state (p = 0.014; Fig. 4).
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CEO of Professional Science Editing for Scientists @ prosciediting.com