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Then, xylose-fed-batch cultivation was performed to induce PaXynG production by adding fed medium [0.2% yeast extract, 0.085% YNB w/o amino acid and ammonium sulfate (Difco) and 20% xylose] at a feeding rate of 500 ml/day using a peristaltic pump.
Nitrogen intake did not differ (P=0.50) between lambs fed MEDIUM (29.8 g/d) and OSC (28.6 g/d) diets.
When compared to those fed MEDIUM (7.9 g/d), lambs fed OSC (9.0 g/d) tended (P=0.09) to have a higher microbial NAN supply.
Compared to PC, feed conversion ratios (FCR) were reduced in growers fed medium or high Ca reduction and high phytase dose diets (P < 0.05).
Retained N in lambs fed OSC (13.6 g/d) was higher (P=0.003) than in lambs fed MEDIUM (9.6 g/d).
Urinary urea-N increased linearly (P<0.001) from LOW (2.3 g/d) to HIGH (6.5 g/d), and lambs fed MEDIUM excreted more (P=0.001) urea-N in urine than those fed OSC.
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The composition of the fed-batch feeding medium was as follows (per liter deionized water): glucose 500 g/L and 1 ml PTM4 trace salts solution.
The feeding medium for fed-batch experiments was composed of 500 g/L glucose.
Feeding medium oat silage increased milk fat percentage and yield compared with feeding a mixture of long and fine oat silage.
These include problems such as the safety margin of the design parameters of the fans and pumps, intensity difference in the feeding medium and cavitation erosion.
The starting flow rate of feeding medium was 1 mL min−1 (dilution rate, D = 0.04 h−1).
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