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Cultures were fed by adding HB101 to a final concentration of 40 mg/ml and 5 larvae/µl.
On Tuesday, however, the Huffington Post reported that, on a close reading, his amendment would in fact decrease transparency at the Fed by adding additional restrictions.
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We induced rhANXA5 expression in fed-batch cultivations with linear ascending feeding by adding IPTG to a final concentration of 1 mM.
For the cell challenges, VLPs were adsorbed by spinoculation at 150 g for 30 min at room temperature (r.t).. Afterwards, the cell cultures were re-fed by adding fresh medium, and incubated at 37°C.
When necessary, cells were fed weekly by adding the supplementary factors listed above (FGF 20 ng/mL, EGF 20 ng/mL, heparin 2 μg/mL).
A user subscribes to an RSS feed by adding it to a list in so-called newsreader software, which includes mobile and desktop programs and web apps like Google Reader.
In another episode Pig, a television chef, feeds a crowd by adding an S to his P-I-E.
Some men try to feed their vanity by adding distinctive goatees, sideburns or even makeup to their faces.
A greater threat than the budget deficit right now is in any case the avalanche of job losses already creating havoc with people's lives across the country, feeding the recession by adding to the banks' bad debts and squeezing spending power still further.
Tadpoles of set two (Sweden) were fed every other day ad libitum with rabbit chow (high food treatment); those of set three in Zürich were fed twice a week by adding to each tub 0.5 g rabbit chow (medium food), while those of set four in Zürich could only feed on algae growing naturally in the tub (low food).
Then, xylose-fed-batch cultivation was performed to induce PaXynG production by adding fed medium [0.2% yeast extract, 0.085% YNB w/o amino acid and ammonium sulfate (Difco) and 20% xylose] at a feeding rate of 500 ml/day using a peristaltic pump.
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