The adult worms were then identified by their morphological features, divided into groups and placed in 24-well plates contained cultivated media RPMI-1640 plus 20% FBS, pH 7.4, in an atmosphere of 95%O2/5%% CO2, 37°C.
The larvae were then identified by morphological features, divided into groups and placed in 24-well plates contained cultivated media RPMI-1640 plus 20% FBS, pH 4.0, in an atmosphere of 95%O2/5%% CO2, 37°C.
In Table 1, we describe these clinical features divided into six categories with the percentage of patients exhibiting each feature, divided into early and later categories (see below).
In Hierarchical approach, face features research is divided in two steps: a rough research of face key points and then a refined analysis of face feature by the mean of a specific model for each face feature (eyes, nose, mouth) [36 39].
SNAREs are essential for eukaryotic vesicle trafficking and according to structural features SNAREs are divided in R (arginine)- and Q (glutamine -SNAREs (Martens and McMahon, 2008).
These features can be divided in four different categories.
For instance, the feature MAYOUNG contained the mRNA absolute expression data from the experiments of Young et al [32], and feature MAYOUNG were divided in to 10 bin(number 45 to 55), with 55 representing no feature record for one protein, and 44 to 54 corresponded to mRNA absolute expression data from low to high.
The gene expression values were quantified in terms of reads per million (RPKM) defined as the total number of reads mapping to the feature divided by feature length (in kbp) normalized by the total number of reads (in millions) [ 68].
The features evaluated can be roughly divided in methods operating in the spatial domain and those operating in the transform domain.
The approaches to assay for CT can be divided in terms of features to be detected: (1) bioassay including rabbit ileal loop test, rabbit skin test, and cultured CHO cell assay, (2) immunoassay including ELISA and RPLA, and (3) DNA-based assay including PCR, qPCR, DNA hybridization, and LAMP [ 3, 4].
