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The CDS product feature qualifier "hypothetical protein" was deliberately excluded as it would be expected to map to different clusters.
Each CDS feature in a GenBank-style annotation record (such as the ones available from the RAST system) typically has a product feature qualifier value associated with it.
Thus, a query of the CDS product feature qualifier values for the term phage anywhere in the product name yielded a mapping of the CDS annotations to 7 core, 190 distributed, and 23 unique clusters.
Table 4 shows that 96% (3,038 of 3,155) of the orthologous clusters generated by our CGS supragenome analysis pipeline[ 21] were comprised of RAST-identified CDS that all mapped to a single product feature qualifier value.
The difference between the results in Tables 5 and 4 indicate that the reverse mapping (of CDS product feature qualifier values to gene clusters) is slightly more susceptible to the problems cited above concerning imprecise CDS product descriptions.
Features can be coloured via the GUI or if the annotation file already has colour information, such as those that can be assigned using Artemis (Rutherford et al., 2000), each feature will be individually coloured according to the input file (i.e. using the feature qualifier : /colour=).
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*Shown are the numbers of orthologous clusters of core, distributed, or unique type whose constituent CDS yield the indicated numbers of distinct RAST annotation CDS "Product" feature qualifiers.
In this analysis 82% (258 of 316) of the RAST annotation CDS product feature qualifiers that describe genes belonging to distributed clusters appeared in only a single cluster, and 91% (1,473 of 1,623) of the core cluster-derived CDS product feature qualifiers were found in only a single cluster.
*Shown are the numbers of distinct RAST annotation CDS "product" feature qualifiers that describe CDS belonging to either core, distributed, or unique clusters and where the relevant CDS yield the indicated number of distinct clusters.
The reverse mapping--of S. aureus RAST annotation product feature qualifiers to CGS-identified CDS clusters--was expected to be more problematic, but these results were also very robust (Table 5).
The fully-functional hierarchical tree of feature and qualifier objects within sequence objects is retained.
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