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Some birds, in which the gland is small or absent, have a specialized type of feather (powder down) that grows continuously and breaks down into a fine powder, believed to be used in dressing the plumage.
The control experiment consisted of buffer and feather powder only.
About 0.5 ml of crude enzyme was incubated with 1.5 g of feather powder suspended in 2 ml of phosphate buffer (pH 7.5).
Similar result was found for Arthrobacter creatinolyticus KP015744 that gave highest keratinase production in presence 1% feather powder (Kate and Pethe 2014).
With feather powder as the substrate, the highest KerP activity was observed at pH 9.0, (Fig. 1a) and the enzyme retained complete activity at pH 7 8 after 48 h of storage at 4 °C.
The medium used for isolation consisted of the following in g/L: NaNO3, 2; NaCl, 2; KH2PO4, 2; MgSO4, 0.05; FeSO4.7H2O, 0.1; CaCO3, 0.1; feather powder, 20; and agar agar, 20 (BDH, UK).
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The enzyme activity was assayed with feather powders as the substrate at 60 °C for 1 h.
The enzyme activity was assayed with feather powders as the substrate at pH 9.0 for 1 h.
callens17 Too much hype is written about the legend of Corbet's Couloir that it overshadows Jackson Hole's true attraction: the unbridled joy of finding your own way down Rendezvous Mountain through a series of waist deep, feather light powder bowls.
Selected keratinase positive bacteria were further confirmed by using feather meal powder in the medium instead of keratin.
Maximum production was 26.57 U/ml in presence of 1.0% keratin in media and keratin-based substrates such as 1.0% feather meal powder gave yield of 16.40 U/ml which is second higher production (Fig. 3).
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