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feather medium.
The keratinolytic activity of mutant strain CC-ZG207 increased by approximately twofold during growth in liquid feather medium.
Previous screening of significant independent variables revealed that concentration of feathers, MgSO4 and KH2PO4 was also an influential factor for the release of amino acids in cultures of K. rhizophila p3-3, grown in feather medium (Table 6).
The microorganism was grown on a feather medium, pH 8.0 (1% feathers) and supplemented with 0.01% of yeast extract, for 5 days, at 28°C with agitation.
The inoculums (10cell/ml) were added to 1 L of feather medium (yeast extract 0.01%, KCl 2.0% and supplemented with chicken feather 1%).
In a thermophilic Meiothermus ruber H328, the MALDI TOF analysis of solubilized products after growth on feather medium detected only oligopeptides with less than 1,000 Daltons [ 27].
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Whole-feather medium (WFM), which contained whole-chicken feather (WCF) 10 g, peptone 5 g, beef extract 5 g, K2HPO4 1 g, MgSO4·7H2O 0.5 g, CaCl2 0.5 g, Na2CO3 5 g, NaCl 5 g, and pH 8.0 was used for protease production.
Each 5-g sample was suspended in 100 mL feather defined medium (defined medium containing 10 g of feathers) in a 500-mL shake flask and incubated at 37 °C at 150 rpm.
After 12 days of culturing in the feather meal medium, complete decomposition of feathers was observed only in flask B4.
Moreover, this recombinant strain could digest the feathers completely in feather defined medium after 7 days of incubation.
The halo-forming colonies thus formed were considered potential candidates for keratinase production, and inoculated into feather defined medium at 37 °C at 150 rpm.
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